West Nile Virus Safety Training for Field and Lab Projects

 

Most of the information in this document was taken from the CDC West Nile Virus Home Page (http://www.cdc.gov/ncidod/dvbid/westnile).

 

WNV Infection in Humans

The most likely route of WNV infection to humans is through the bite of an infected mosquito. Most cases of WNV are probably not acquired in the workplace but there are some workers at risk of WNV infection, such as people who work outside.  Workers who perform necropsies of infected birds or handle WNV-infected tissues or fluids are also at risk of WNV infection if skin penetration or laceration occurs.  There is no evidence that a person can get the virus from handling live or dead infected birds (CDC). WNV may also be transmitted by organ transplantation, blood transfusion, or possibly breast milk [CDC 2002a,b].  Laboratory-acquired infection has also been documented [CDC 2002d]. Turkey breeder farm workers have been infected with WNV [CDC 2003]. The mode of transmission to these farm workers is uncertain.

 

Most human WNV infections cause either no symptoms or a mild flu-like illness. The most severely affected patients may develop an inflammation of the brain, or the membranes of the brain or spinal cord, or both, called encephalitis, meningitis, or meningoencephalitis, respectively. These severe cases may be fatal. Persons over age 50 are at higher risk of severe illness following infection.  All workers should be careful to follow the recommendations listed below even though older workers may be at higher risk of severe disease.  Recovery can take up to a year. Students with immuno-deficiencies should not handle animals at any time.

 

What are the signs and symptoms of WNV infection in humans?

Most human WNV infections cause either no symptoms or a mild flu-like illness. Mild symptoms include fever, fatigue, headache, and muscle or joint pain. Signs of severe infection include high fever, stiff neck, disorientation, tremors, muscle weakness, and paralysis. The most severely affected patients may develop an inflammation of the brain, or the membranes of the brain or spinal cord, or both, called encephalitis, meningitis, or meningoencephalitis, respectively. The incidence of severe disease is highest among persons over age 50. These severe cases may be fatal. The time of incubation from mosquito bite to clinical symptoms is reported to be from 3 to 14 days.

 

Is a woman's pregnancy at risk if she is infected with WNV?

There is one documented case of transplacental (mother-to-fetus) transmission of WNV in humans (CDC 2002c).  . The newborn in this case was infected with WNV at birth and had severe medical problems. It is unknown whether the WNV infection itself caused these problems. More research is needed to understand the possible effects of WNV on pregnancy.  Pregnant women should take precautions to reduce their risk for WNV infection by avoiding mosquitoes, wearing protective clothing and using repellents according to their Doctor’s recommendations.

 

What should a project worker do who suspects he or she has been infected with WNV?

Any worker who has health concerns should notify his or her supervisor.  Unpaid project workers (non-employees) should contact the Student Health Center or their own Medical Care Provider if they have one.  University or Foundation-paid workers should notify their supervisor and the CNSM Safety Office, then proceed to the appropriate Medical Care Facility (see “Injured Employee Guide” on the CNSM Safety Website, or CNSM Safety Memo 61 in the CNSM Safety Manual).  No specific treatment exists for WNV infection. Treatment consists of supportive care for the individual. Currently, no approved vaccine exists to prevent WNV infection in humans.

 

 

Guidelines for Protecting Workers from West Nile Virus Exposure

 

 

Recommendations for Field (Outdoor) Workers

The most likely way persons become infected with WNV is through the bite of an infected mosquito.

Outdoor workers can decrease their risk of WNV infection by reducing their contact with mosquitoes through the use of the personal protective measures listed below. Information about exposure to insecticides can be found at http://www.cdc.gov/ncidod/dvbid/westnile/qa/insect_repellent.htm.

 

Workers should take the following steps when working at sites where mosquitoes may be actively biting:

·        Wear long-sleeved shirts, long pants, and socks when possible.

·        Consider applying insect repellent to exposed skin, as appropriate and according to label directions.

·        Do not apply pump or aerosol products directly to the face; protect eyes at all times. These products should be sprayed onto the hands and then carefully patted onto the face, avoiding the eyes and mouth.

·        DEET (N,N-diethyl-m-toluamide or N,N-diethyl-3-methylbenzamide) is the most effective insect repellent available. The more DEET a repellent contains the longer time it will be effective.  DEET concentrations higher than 50% do not increase the length of protection.

·        For details regarding the safety of DEET, see http://www.nmnh.si.edu/BIRDNET/WNV.html#append

·        If you use repellents, apply at the lowest effective concentration.

·        Do not apply repellents to cuts, wounds, or irritated skin.

·        Wash treated skin with soap and water after returning indoors.

·        Clothing can be treated with products containing DEET or permethrin, as mosquitoes may bite through thin clothing, although it may ruin some fabrics.

·        Permethrin should only be used on clothing; do not apply it directly to skin.

·        Wash treated clothing before wearing it again.

·        Do not apply repellent to skin that is under clothing.

·        Report in a timely manner all injuries and illnesses of suspected WNV origin.

 

Requirements for Workers Handling Dead or Live Birds

·        Use personal protective equipment that provides barrier protection as appropriate including gloves, labcoats, chemical splash goggles if potentially contaminated fluids are present etc.  Appropriate gloves provide a protective barrier that prevents blood and other body fluids from passing through them.

·        Wash hands and other appropriate skin surfaces with soap and water after handling birds and immediately after contact with blood, feces etc., and after removing gloves and before leaving the workplace.  Workers are at risk of WNV infection if their skin is penetrated or cut while performing necropsies or handling WNV-infected tissues or fluids [CDC 2002c].

·        Minimize the generation of aerosols.  If aerosols may be created, use of a Biological Cabinet (Biohood) is recommended.

·        Handle sharp instruments carefully during use.

·        Use medical devices with safety features when available to avoid sharps-related injuries.

·        Never bend, break or recap needles.

·        Dispose of sharp instruments carefully after use in the appropriate rigid sharps container; obtain these free containers from CNSM Safety.

·        Report to the supervisor all needlestick and other sharps-related injuries.

·        Report to the supervisor any laboratory incidents or accidents involving possible WNV exposure.

·        Report to the supervisor any symptoms consistent with WNV infection.

 

Additional Requirements for Workers in the CSULB Animal Care Facility

·        Use a  labcoat provided by the Animal Care Facility while working with the birds.  Remove this labcoat and return it to the storage location before exiting the Facility. 

·        Do not wear your own labcoat into the facility as this may bring unwanted contaminants into the animal care area.

The above are basic recommendations to reduce exposure to blood and other tissues from WNV-infected animals or persons.  More stringent protective equipment and work practices should be used when warranted; consult CNSM Safety for details.  Biosafety guidelines are available for working with WNV and other microbiological agents in the laboratory; see CDC/NIH Guidelines for “Biosafety in Microbiological and Biomedical Laboratories” (http://www.cdc.gov/od/ohs/biosfty/bmbl4/bmbl4toc.htm).

 

Links to other Sites with WNV Information

 

U.S. Government Web sites

 

CDC West Nile Virus Home Page
http://www.cdc.gov/ncidod/dvbid/westnile

 

U.S. Department of Agriculture Animal and Plant Health Inspection Service, West Nile Virus Site
http://www.aphis.usda.gov/lpa/issues/wnv/wnv.html

 

CDC/NIH Guidelines; “Biosafety in Microbiological and Biomedical Laboratories  (BMBL) 4th Edition”

http://www.cdc.gov/od/ohs/biosfty/bmbl4/bmbl4toc.htm

 

U.S. Geological Survey National Wildlife Health Center, West Nile Virus Site
http://www.nwhc.usgs.gov/research/west_nile/west_nile.html

 

National Institutes of Health, National Institute of Allergy and Infectious Diseases, West Nile Virus Site
http://www.niaid.nih.gov/publications/wnile/

 

Food and Drug Administration, West Nile Virus Site
http://www.fda.gov/oc/opacom/hottopics/westnile.html

 

State and Local Government Web sites

City of Long Beach Vector Control,

http://www.ci.long-beach.ca.us/health/organization/eh/vector/default.asp

 

State and Local Government Web site links on the CDC West Nile Virus Home Page
http://www.cdc.gov/ncidod/dvbid/westnile

 

Other Web sites

 

Cornell University Environmental Risk Analysis Program, WNV Section
http://www.cfe.cornell.edu/erap/wnv

 

National Library of Medicine, Medlineplus West Nile Virus Site
http://www.nlm.nih.gov/medlineplus/westnilevirus.html

 

National Pesticide Information Center Web Site, West Nile Virus Resource Guide
http://npic.orst.edu/wnv

 

CNSM Safety Website, http://www.cnsm.csulb.edu/services/safety/index.htm

 

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